Immunofluorescence:Article Title: Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells
Article Snippet: .. Primary antibodies used for immunofluorescence and immunohistochemistry include the following: NKX6.1 (DSHB, F55A12-c, 1:300), PDX1 (R&D, AF2419, 1:300), INS-APC (Cell Signaling, C27C9, 1:80), NKX6.1-PE (BD, #563023, 1:40), Human C-peptide (Abcam, Ab14181, 1:100), Glucagon (Cell Signaling, 2760 S, 1:400), Glucagon (Abcam, ab82270, 1:100), Insulin (Abcam, ab7842, 1:100), GFP (Abcam, ab6673, 1:300), NEUROD1 (R&D, AF2746, 1:200), and NEUROD1-APC (BD, 563000, 1:50). .. Secondary antibodies used were as follows: Donkey anti-mouse Alexa Fluor 488 (Jackson Lab, 715-545-151), Donkey anti-goat Alexa Fluor 488 (Jackson Lab, 705-545-147), Donkey anti-rabbit Alexa Fluor 488 (Jackson Lab, 711-545-152), Donkey anti-guinea pig Alexa Fluor 488 (Jackson Lab, 706-545-148), Donkey anti-mouse Alexa Fluor 550 (Invitrogen, SA5-10167), Donkey anti-rat Alexa Fluor 555 (Abcam, ab150154), Donkey anti-rabbit Alexa Fluor Cy3 (Jackson Lab, 711-165-152), Donkey anti-rat Alexa Fluor 647 (Abcam, ab150155), Donkey anti-goat Alexa Fluor 647 (Jackson Lab, 705-605-147), Donkey anti-guinea pig Alexa Fluor 647 (Jackson Lab, 706-605-148), Donkey anti-mouse Alexa Fluor 647 (Jackson Lab, 715-605-151), Donkey anti-guinea pig FITC (Jackson Lab, 706-095-148), Donkey anti-mouse Cy5 (Jackson Lab, 715-175-151), Donkey anti-mouse Alexa Fluor 647 (AF647) (Jackson Lab, 715-605-151), and Donkey anti-rabbit Alexa Fluor 647 (Jackson Lab, 711-605-152).
Article Title: Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells.
Article Snippet: .. Primary antibodies used for immunofluorescence and immunohistochemistry include the following: NKX6.1 (DSHB, F55A12-c, 1:300), PDX1 (R&D, AF2419, 1:300), INS-APC (Cell Signaling, C27C9, 1:80), NKX6.1-PE (BD, #563023, 1:40), Human C-peptide (Abcam, Ab14181, 1:100), Glucagon (Cell Signaling, 2760 S, 1:400), Glucagon (Abcam, ab82270, 1:100), Insulin (Abcam, ab7842, 1:100), GFP (Abcam, ab6673, 1:300), NEUROD1 (R&D, AF2746, 1:200), and NEUROD1-APC (BD, 563000, 1:50). .. Cells were incubated with primary antibodies at 4 °C overnight, followed with three rinses, and an incubation with secondary antibodies at room temperature for 1 h. The stained cells were rinsed with PBS and then incubated with DAPI (Sigma-Aldrich) for 2 min to stain the nuclei.
Immunohistochemistry:Article Title: Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells
Article Snippet: .. Primary antibodies used for immunofluorescence and immunohistochemistry include the following: NKX6.1 (DSHB, F55A12-c, 1:300), PDX1 (R&D, AF2419, 1:300), INS-APC (Cell Signaling, C27C9, 1:80), NKX6.1-PE (BD, #563023, 1:40), Human C-peptide (Abcam, Ab14181, 1:100), Glucagon (Cell Signaling, 2760 S, 1:400), Glucagon (Abcam, ab82270, 1:100), Insulin (Abcam, ab7842, 1:100), GFP (Abcam, ab6673, 1:300), NEUROD1 (R&D, AF2746, 1:200), and NEUROD1-APC (BD, 563000, 1:50). .. Secondary antibodies used were as follows: Donkey anti-mouse Alexa Fluor 488 (Jackson Lab, 715-545-151), Donkey anti-goat Alexa Fluor 488 (Jackson Lab, 705-545-147), Donkey anti-rabbit Alexa Fluor 488 (Jackson Lab, 711-545-152), Donkey anti-guinea pig Alexa Fluor 488 (Jackson Lab, 706-545-148), Donkey anti-mouse Alexa Fluor 550 (Invitrogen, SA5-10167), Donkey anti-rat Alexa Fluor 555 (Abcam, ab150154), Donkey anti-rabbit Alexa Fluor Cy3 (Jackson Lab, 711-165-152), Donkey anti-rat Alexa Fluor 647 (Abcam, ab150155), Donkey anti-goat Alexa Fluor 647 (Jackson Lab, 705-605-147), Donkey anti-guinea pig Alexa Fluor 647 (Jackson Lab, 706-605-148), Donkey anti-mouse Alexa Fluor 647 (Jackson Lab, 715-605-151), Donkey anti-guinea pig FITC (Jackson Lab, 706-095-148), Donkey anti-mouse Cy5 (Jackson Lab, 715-175-151), Donkey anti-mouse Alexa Fluor 647 (AF647) (Jackson Lab, 715-605-151), and Donkey anti-rabbit Alexa Fluor 647 (Jackson Lab, 711-605-152).
Article Title: Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells.
Article Snippet: .. Primary antibodies used for immunofluorescence and immunohistochemistry include the following: NKX6.1 (DSHB, F55A12-c, 1:300), PDX1 (R&D, AF2419, 1:300), INS-APC (Cell Signaling, C27C9, 1:80), NKX6.1-PE (BD, #563023, 1:40), Human C-peptide (Abcam, Ab14181, 1:100), Glucagon (Cell Signaling, 2760 S, 1:400), Glucagon (Abcam, ab82270, 1:100), Insulin (Abcam, ab7842, 1:100), GFP (Abcam, ab6673, 1:300), NEUROD1 (R&D, AF2746, 1:200), and NEUROD1-APC (BD, 563000, 1:50). .. Cells were incubated with primary antibodies at 4 °C overnight, followed with three rinses, and an incubation with secondary antibodies at room temperature for 1 h. The stained cells were rinsed with PBS and then incubated with DAPI (Sigma-Aldrich) for 2 min to stain the nuclei.
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